![]() ( I) Representative images of GFAP staining of cerebellar astrocytes from P60 mice of genotypes indicated. ( H) The DLK protein levels in cerebellum of P60 mice of genotypes indicated were determined by western blot. ( G) The homozygotes or heterozygotes of Map3k13 fl were identified by PCR using primers YJ12525 and YJ12526. ( F) Map3k13 fl/fl mice have loxP sites flanking exon 2, and Cre-mediated excision results in frameshift, hence knockout of LZK. ( E) The homozygotes or heterozygotes of Map3k12 fl were identified by PCR using primers YJ12523 and YJ12524. ( D) Map3k12 fl/fl mice have loxP sites flanking the exon 4 that contains the initiation ATG codon, and Cre-mediated excision results in knockout of DLK. ( C) The LZK protein levels in cerebellum of P60 mice of genotypes indicated were determined by immunoprecipitation and western blot. ( B) The homozygotes or heterozygotes of Map3k13 KO were identified by PCR using primers YJ12529-12531. ( A) Map3k13 KO mice were generated by CRISPR-Cas9 technology using three sgRNAs targeted to the kinase domain of LZK.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |